Abstract
This study examined the potential effect of HAI-1 over-expression on autophagy of prostate cancer PC-3 cells and its possible mechanism. PC-3 cells were transfected with plasmids to generate HAI-1 stably over-expressing PC-3/HAI-1 and control cell lines. The relative levels of HAI-1 mRNA transcripts in control PC-3 and PC-3/HAI-1 cells were determined by RT_PCR. The levels of Beclin1 expression in both cell lines were determined by Western blot. The levels of autophagic vesicles and autophagosomes in these cell lines were characterized by Cyto-ID® Autophagy Detection Dye Staining and transmission electronic microscopy. In comparison with the control PC-3 cells, HAI-1 mRNA transcripts and Beclin1 expression significantly increased in PC_3/HAI1 cells. There were many autophagic vesicles and autophagosomes detected in PC-3/HAI-1 cells. In conclusion, HAI-1 over-expression may promote autophagy of prostate cancer PC-3 cells in vitro.
For More Details: http://www.sciaeon.org/articles/HAI-1-Over-Expression-Promotes-Autophagy-of-Prostate-Cancer-PC-3-Cells.pdf
Journal Information: http://www.sciaeon.org/cancer-biology-and-clinical-oncology/home
Manuscript Submission: http://www.sciaeon.org/submit-paper
Contact: cancerbiology@sciaeonopenaccess.com
Journal of Cancer Biology and Clinical Oncology 